Table 1. Percentage of T0 transgenic plants examined with mutations and GC content of sgRNAs.

Vector	Target gene	sgRNA	No. of lines	No. of lines with mutations	Mutation rate	sgRNA GC content
AtU6-sgRNA-AtUBQ-Cas9	SlPDS	sgRNA1	22	16	72.70%	40.00%
	SlPDS	sgRNA2	7	7	100.00%	55.00%
AtU6-sgRNA-2 × CaMV 35S-Cas9	SlPIF4	sgRNA1	25	21	84.00%	60.00%
	SlPIF4	sgRNA2	19	17	89.47%	50.00%
Total	2	4	73	61	83.56%	51.25%