Table 3. Segregation patterns of CRISPR/Cas9-medicated targeted mutagenesis during the T0 to T1 generation.
| Target gene | sgRNA | Line# | T0 |
T1 |
||
|---|---|---|---|---|---|---|
| Zygosity$ | Genotype | Mutation segregation | T-DNA | |||
| SlPIF4 | 1 | T0-22 | Homozygote | d1d1 | 15d1d1 | 12+;3− |
| SlPIF4 | 2 | T0-19 | Homozygote | d1d1 | 14d1d1 | 10+:4− |
| SlPIF4 | 2 | T0-8 | Biallele | d2,d9 | 3d2d2,6e*,5d9d9 | 10+:4− |
| SlPIF4 | 2 | T0-10 | Biallele | d3,i1 | 1d3d3,7e,5i1i1 | 8+:5− |
| SlPDS | 1 | T0-20 | Chimera | d5,d12,c102,WT | 8e | 5+;1− |
| SlPIF4 | 1 | T0-3 | Chimera | d3,d4,i1 | 13e,2i1 | 11+:4− |
| SlPIF4 | 2 | T0-12 | Chimera | d2,d6,d17,c7 | 5d6,2e,5d2 | All+ |
| SlPIF4 | 2 | T0-16 | Chimera | d1,d2,d3 | 8d2,11e,3d1 | 14+:8− |
| SlPIF4 | 1 | T0-6 | WT | WT | 13WT | 12+:1− |
| SlPDS | 1 | T0-18 | WT | WT | 16WT | 9+:7− |
#Line name is in the format of T0-#.
$The zygosoty of homozygote, biallele and chimera in T0 plant lines were putative. d#, # of bp deleted at the target sites; i#, # number of bases insertion at target sites; c#, combined mutation; WT, wild-type sequence without mutations detected at target sites.
*e, heterogeneous, more than one sequence detected in the sample; +, T-DNA was detected; −, T-DNA was not detected.