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. 2016 Apr 21;6:24779. doi: 10.1038/srep24779

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(a,b) Representative H&E staining of the ultrastructure of native liver and decellularized liver matrix. Scale bars = 100 μm. DAPI staining (c,d) and Sirius Red staining (e,f) illustrate a lack of residual cellular components and the preservation of matrix components in the decellularized ECM. Scale bars = 200 μm. Immunohistochemical staining of native and decellularized liver tissues for α-Gal (g,h), SLA-2 (i,j), SLA-DRα (k,l) are shown. Scale bars = 100 μm. (m) Semi-quantitative PCR for the α-Gal, SLA-DRα, PERV, and SLA-2 antigens in the liver scaffolds. Na = native liver; De = decellularized liver matrix.