Figure 3. Generation of anti-PS-I mAbs.

(a) Mice were immunized with pentasaccharide 1-CRM₁₉₇ glycoconjugate⁷ at indicated time points. Sera were collected for glycan microarray-assisted evaluation of the immune responses. Splenocytes of one mouse were subjected to hybridoma cell fusion²⁷. (b) Spotting pattern of glycan microarrays presented in c and d. Buffer was 50 mM sodium phosphate, pH 8.5. Compound 1 was spotted at 0.1, 0.5 and 1 mM; CRM₁₉₇ and the spacer dummy was spotted at 0.1, 0.5 and 1 μM; and 7 and 8 both at 1 mM. (c) Microarray scans representing the serum IgG response of one mouse. See Supplementary Fig. 1 for details. (d) Representative microarray scans of hybridoma cell supernatants of clone 5A9 producing antibodies to the spacer and 2C5 producing antibodies to PS-I.