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. 2016 Apr 19;7:11310. doi: 10.1038/ncomms11310

Figure 6. Genome-wide enrichment of HP1β correlates with SETDB1-dependent H3K9me3 deposition.

Figure 6

(a) Plot of genome-wide correlation of mHP1β and H3K9me3 (ref. 35) as deduced by ChIP-seq in mouse ESC and using 1 kb-sized windows. Dashed line indicates the data trend computed by loss regression. (b) Tracks displaying number of library-normalized reads per 100 bp from the indicated ChIP-seq experiments. Data obtained from antibody-based enrichments of endogenous mHP1β (top) and GFP-tagged mHP1β (middle) and the respective input tracks are shown. H3K9me3 data in SETDB1f/− and SETDB1−/− experiments are from ref. 36. Gene models and repetitive elements are indicated at the bottom. (c) Analysis as in a but comparing mHP1β binding to H3K9me3 loss in SETDB1−/− mESC. H3K9me3 loss is shown as the log2-difference between SETDB1f/− and SETDB1−/−.