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. 2016 Apr 19;7:11389. doi: 10.1038/ncomms11389

Figure 2. MT dynamics at the IS is impaired by Aurora A chemical inhibition.

Figure 2

(a–d) Imaging of EB3-GFP-expressing CH7C17 T cells (pretreated with DMSO or MLN8237 and settled on corresponding anti-CD3/CD28-coated glass-bottom chambers). Maximal projection of XYZ stacks for fluorescence and single bright-field (BF) images are shown. Scale bar, 10 μm. (b) Ratio of EB3-GFP fluorescence incorporated in +tips from XYZ stacks (0 s; n=34 in DMSO and n=43 in MLN8237). Data represent means±s.d. Means were compared with a Mann–Whitney test. (c) Orthogonal and 3D projections from XYZ stacks. Dotted white or red lines indicate contact with substrate or media, respectively. (d) Ratio of the MTOC location from the IS-like structure (n=38 in DMSO, n=44 in MLN8237). (e) Distance from the T-cell MTOC to the APC contact area in conjugates of T cells with SEE-pulsed APCs (10 min, n=166 in DMSO, n=168 in MLN8237; 30 min, n=412 in DMSO, n=394 in MLN8237). Data represent means±s.d. from three independent experiments. Means were compared with a t-test.