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. 2016 Apr 19;7:11389. doi: 10.1038/ncomms11389

Figure 7. Aurora A inhibition impairs TCR signalling and gene expression.

Figure 7

(a) Immunoblottings showing phosphorylation of the molecules indicated in lysates of CH7C17 Jurkat T cells pretreated with DMSO or MLN8237 and conjugated for the indicated times with HA-peptide-pulsed Hom2 B cells. (b) Quantification of blots as in a–c from four to six independent experiments. Error bars represent interquartile range. Medians were compared with a Friedman test (*P<0.05). n.s., nonsignificant. (c) IL2, CD69 and CD25 mRNA levels in primary human CD4+ T cells pretreated with DMSO, MLN8237 (10 μM) or the Aurora B inhibitor AZD1152 (100 nM) and activated by settling on anti-CD3/CD28-coated plates for 4 h. mRNA levels were normalized to the housekeeping gene GAPDH and the levels of the target mRNA in non-stimulated cell levels. Error bars represent interquartile rage. Medians were compared with a Mann–Whitney test. **P<0.01.