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. 2016 Feb 25;1(2):e85817. doi: 10.1172/jci.insight.85817

Figure 5. Doxorubicin-induced mitochondrial abnormalities at day 60.

Figure 5

Transmission electron micrographs of the left ventricle (LV) 60 days after treatment with (A–C) vehicle or (D–F) doxorubicin (DOX) in (A and D) WT mice, (B and E) mice with cardiac-specific overexpression of heme oxygenase-1 (cs-HO-1), and (C and F) HO-1 knockout (HO-1–/–) mice. White Vs mark nonmembrane-bound vacuoles, and white arrows mark mitochondria engaged with a membrane-bound vacuole. Original magnification, ×4,400. Scale bar: 2 μm. (G) Mitochondrial number and (H) area were quantified on images collected at an original magnification of ×1,650. Image evaluation and mitochondrial quantification were performed by a reviewer blinded to the different groups (n = 3–5 mice per group) and averaged from at least 5 images per mouse. Data presented as mean ± SEM. P < 0.05, ANOVA and the Newman-Keuls post-test were used to determine statistically significant changes.