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Translocation of BCCP and biotin during ACC catalysis. The BC and CT active sites (black asterisks) of ScACC are separated by ~80 Å (arrow). A rotation of ~180° around the BT-BCCP linker (curved arrow in blue) could place the biotin into the BC active site. The prime in the labels indicates the second protomer. The binding modes of ADP (green) and biotin (black) to E. coli BC subunit are shown as stick models26.
