Fig. 2.

Transcript levels of GATA-1 and KLF1 in maturing erythroid cells derived from CD34⁺ cells of independent ontology. Erythroid cells derived from fetal liver or adult bone marrow CD34⁺ cells exposed to identical conditions were collected during early, intermediate (mid), or late stages of differentiation. Cells were isolated of total RNA and levels of GATA-1 and KLF1 transcripts determined by quantitative RT-PCR. Data were normalized to RNAseP, set relative to fetal liver, and plotted as mean+SD; n=2 independent donors performed in duplicate.