Table 2.
Analysis of variance for prunasin concentration in roots of Prunus serotina seedlings acclimated to one of three light regimes: 10 % low light (LL), 25 % medium light (ML), or 100 % high light (HL) of full natural sun light. Samples were taken on three occasions: in June (DOY 176, DOY – day of year), in August (DOY 238), and in September (DOY 273). The split-split plot model of ANOVA was applied with block, time of sampling, light treatment, combination, and interactions as the sources of variance. The differences were significant at P ≤ 0.05. MS – mean sum of squares, F – value of Snedecor’s function, in bold – statistically significant at P < 0.05 (N = 106, N – number of seedlings)
| Source of variance | Effect | Degrees of freedom | MS | F | P |
|---|---|---|---|---|---|
| Block | Random | 2 | 0.22 | 0.21 | 0.065 |
| Time of sampling | Fixed | 2 | 28.70 | 26.95 | 0.005 |
| Error I (Block × Time of sampling) | Random | 4 | 1.07 | 0.67 | 0.626 |
| Light | Fixed | 2 | 6.42 | 4.03 | 0.045 |
| Time of sampling × Light | Fixed | 4 | 13.58 | 8.53 | 0.002 |
| Error II (Block × Light + Block × Time × Light) | Random | 12 | 1.59 | 1.13 | 0.401 |
| Competition with mulching | Fixed | 1 | 0.27 | 0.19 | 0.666 |
| Time of sampling × Competition with mulching | Fixed | 2 | 0.58 | 0.411 | 0.669 |
| Light × Competition with mulching | Fixed | 2 | 2.01 | 1.42 | 0.269 |
| Time of sampling × Light × Competition with mul. | Fixed | 4 | 3.55 | 2.51 | 0.081 |
| Error III | Random | 17 | 1.41 | ||
| Total | 52 |