Fig. 3.

Transcriptional responses of ABA core signaling components to ABA, showing time- and dose-dependent patterns in maize primary roots. The root system of 15-day-old seedlings was exposed to 0 (control), 1, 10 or 50 μM ABA for 3 h (a, b, c) or to 1 μM ABA for 0 (control), 0.5, 1 or 3 h (d, e, f). Gene expression of ABA receptors PYR/PYL/RCAR (a, d), PP2C (b, e) and SnRK2s (c, f) was measured in the primary roots by real-time PCR. The results are the means of three biological replicates of four primary roots, each ± SE