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The salinity tolerance of ANT3 cells expressing altered forms of AjSOS1. The yeast cells were cultured overnight and a 5 μL aliquot (serially diluted) was spotted onto either a YPDA medium containing no NaCl (a-c) or an AP medium containing 70 mM NaCl (d-e). Plates were incubated at 30 °C for 2–4 days
