Table 2.

Primers for PCR amplification of the connection region of integron and ISCR1

Type of complex class 1 integron	No.	Primer name	Sequence (5′–3′)
A	I	aadA2 + bla CTX-9F	CGTTGCCTTGGTAGGTCC
	II	aadA2 + bla CTX-9 up R	TTGCTTGCCATAGTCATCTT
	IX	aadA2 + bla CTX-9 down F	CCCCAAGGAGCCCATTC
	X	aadA2 + bla CTX-9R	GGTATTCAGCGTAGGTTCAGT
B	I	aadA2 + qnrA1 F	GTTGTCCCGCATTTGGT
	II	aadA2 + qnrA1 up R	GGTTGAGCGAGAAGGTTTT
	IX	aadA2 + qnrA1 down F	GCGTGAGCTGCCACCAGAA
	X	aadA2 + qnrA1 R	TCTTATGGCTGACTTGATTGTAG
C	I	aadA1 + bla DHA-1 F	ATCTGGCTATCTTGCTGAC
	II	aadA1 + bla DHA-1 up R	TTCCGAGAAGGTGATTGC
	IX	aadA1 + bla DHA-1 down F	CCAACACTGCTCAACACT
	X	aadA1 + bla DHA-1 R	GGTGGCGATTGTGATTCT
D	I	dfrA25+ sapA F	ACGAAGCGATGGGTAGA
	II	dfrA25+ sapA up R	AGCCCTCACGAGTTGTTAT
	IX	dfrA25+ sapA down F	CAAGAAGCCCGACAAAT
	X	dfrA25+ sapA R	TGGGAGGTGCTGGATAA
E	I	aadA2+ sapA F	CGTTGCCTTGGTAGGTC
	II	aadA2+ sapA up R	AACCGCACAATCTCGTC
	IX	aadA2+ sapA down F	CGCTGCTGATAGACGAAG
	X	aadA2+ sapA R	TGGGAGGTGCTGGATAA
F	I	aadA16+ sapA F	GTTGTTCCTTGGCGTTATC
	II	aadA16+ sapA up R	TCAGCAATATCGGGATAGAG
	IX	aadA16+ sapA down F	AGACGATACGCTGACTCA
	X	aadA16+ sapA R	ATGACCGACTGCTTGATG
G	I	dfrA27 + short chain F	GCAATGAGGGAGCTAAAGA
	II	dfrA27 + short chain up R	TTGGGTTCAGGGTGCTAT
	IX	dfrA27 + short chain down F	CAAGAAGCCCGACAAATC
	X	dfrA27 + short chain R	TTCACGAGCATAGGCAATA
H	I	aacA4 + bla PER F	CCCGAGGTCACCAAGA
	II	aacA4 + bla PERup R	GCACCATCCCACATAAGA
	IX	aacA4 + bla PERdown F	AAGAGGGCGAAGACGA
	X	aacA4 + bla PER R	TCCATCAGGCAACAGAAT
I	I	aadA5 + bla PER F	ACTGGTCTCATTGCTCCTA
	II	aadA5 + bla PER up R	CGAAGAACCGCACAATCT
	IX	aadA5 + bla PER down F	CAACACTGCTCAACACTG
	X	aadA5 + bla PER R	ATTGGTTCGGCTTGACTC
J	I	aadA24 + bla PER F	CATCATTCCGTGGCGTTA
	II	aadA24 + bla PER up R	GACACCGAGACCAATAGC
	IX	aadA24 + bla PER down F	AATCCAACACTGCTCAACA
	X	aadA24 + bla PER R	CATCATTCCGTGGCGTTA
K	I	arr-3 + qnrA1 F	GGTAATCCAACACAGTCCTA
	II	arr-3 + qnrA1 up R	GTCCGCCTCAGCAATATC
	IX	arr-3 + qnrA1 down F	TCCAACACTGCTCAACAC
	X	arr-3 + qnrA1 R	CCAGAGTATCCGCAATCC
L	I	bla PSE-1 + dfrA10 F	TTATGGCGGCGTTAGATG
	II	bla PSE-1 + dfrA10 up R	CGAGACCAATAGCGGAAG
	IX	bla PSE-1 + dfrA10 down F	ATATTGAAGTCTGCGAACAC
	X	bla PSE-1 + dfrA10 R	CGTGCTCTGTGATAGTTGA
-	III	common 1 791 F	TATTGCTGAGGCGGACTG
	IV	common 1 791 R	CATTGGAGGAGGTCGTTG
	V	common 2 1054 F	GGCTTCCGCTATTGGTC
	VI	common 2 1054 R	TTGCTTGCCATAGTCATCTT
	VII	common 3 1727 F	TCGCCCACTCAAACAAA
	VIII	common 3 1727 R	GCTCCTCATCCGAAGTATCT
	A	-	CCTGTCGGTGTTGCTTAT
	B	-	GTTGCTTGCCATAGTCATC

Types of complex class 1 integron correspond to Fig. 2;

Primers No. correspond to Fig. 1