Fig. 5.

Compound 6 reduces MCF7 tumorigenesis in vitro and in vivo. a Compound 6 demonstrated a significant concentration dependent reduction in MCF7 invasion from 100 nM (***P<0.001). b MCF7 cells were propagated in BALB/c nude mice and subsequently treated with compound 6 (100 mg/kg) over the course of 29 days, indicated by black arrows. Treatment with compound 6 caused a significant reduction in tumor volume of 31 % versus vehicle by day 29 (**P<0.01). Immunohistochemical analysis of proliferation was carried out on MCF7 tumor sections. c Compound 6 caused a significant 0.62-fold reduction in tumor proliferation (***P<0.001), as demonstrated in the representative images. d TUNEL analysis of apoptosis was carried out on the MCF7 tumor sections. Compound 6 treatment demonstrated a 1.60-fold increase of apoptosis within the tumor sections (***P<0.001), as demonstrated in the representative images