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. Author manuscript; available in PMC: 2016 Nov 18.
Published in final edited form as: Bioconjug Chem. 2015 Jul 24;26(11):2243–2248. doi: 10.1021/acs.bioconjchem.5b00244

Figure 4.

Figure 4

Internalization of dual labeled THIO-SELENOMAB. (A) HER2-positive SK-BR-3 cells were incubated with 20 ng/ml of unlabeled scFv-Fc or dual labeled scFv-FcS396C-Sec/biotin/ODA-fluorescein at 37°C at the indicated time points. The mean fluorescence intensity (MFI) of the cells was measured by flow cytometry using an APC-conjugated goat anti-human Fcγ antibody. (B) SK-BR-3 and MDA-MB-468 cells were incubated with 2 μg/ml dual labeled scFv-FcS396C-Sec/biotin/ODA-fluorescein at 37 °C for 4 h. Internalized antibody was detected by Cy3-conjugated goat anti-human Fcγ antibody. Cell nuclei were stained with Hoechst 33342. DIC represents the image from differential interference contrast.