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. Author manuscript; available in PMC: 2016 Nov 18.
Published in final edited form as: Bioconjug Chem. 2015 Jul 24;26(11):2243–2248. doi: 10.1021/acs.bioconjchem.5b00244

Figure 5.

Figure 5

Stability of dual labeled THIO-SELENOMAB in human plasma. (A) Western blot and SDS-PAGE analysis of scFv-FcS396C-Sec/biotin/ODA-fluorescein in human plasma at 37°C at the indicated time points. HRP-conjugated ExtrAvidin and a mouse anti-His mAb were employed to detect the stability of biotin labeling (top two lanes). Fluorescent and Coomassie-stained gels were used to detect the stability of fluorescein labeling (bottom two lanes). (B) Quantitative analysis of the band intensity in (A) using Image J software.