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. 2016 Feb 23;65(5):1387–1397. doi: 10.2337/db15-0519

Figure 5.

Figure 5

Effects of HIF-1α or GLUT1 knockdown on HG-induced fibronectin expression. A: MCs were exposed to LG or HG (HG for 24 h). RNA was extracted and HIF-1α and GLUT1 mRNA were examined by quantitative real-time PCR (qtPCR). MCs were transfected with scrambled control (SCR) or siHIF-1α (B) or siGLUT1 (C). Expression of fibronectin (FN), HIF-1α, and GLUT1 was evaluated by Western blot. The data are representative of three independent experiments and are expressed as fold control (LG), defined as 1. Values represent mean ± SEM. **P < 0.01 vs. HIF-1α LG. ***P < 0.001 vs. GLUT1 LG.