Figure 7.

GLUT1 mediates fibronectin expression through NOX4. MCs were transfected and exposed to LG or HG 7. Before harvesting, indicated plates were infected with adenovirus GFP (ADGFP) or adenovirus NOX4 (ADNOX4). A and B: Fibronectin (FN) expression was assessed by Western blot analysis. Actin was immunoblotted as a loading control. C: Parallel to A and B, cells were plated on cover slips. Fibronectin was examined by immunofluorescence as outlined in Research Design and Methods. D: NOX4 expression was assessed in MCs infected with adenovirus GFP or adenovirus NOX4. E: Schematic for HIF-1 inhibition blocking progression of DN through reduction of GLUT1-dependent, NOX4-derived oxidative stress in vitro and in vivo. ECM, extracellular matrix; SCR, scrambled control.