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. Author manuscript; available in PMC: 2016 Apr 21.
Published in final edited form as: Nat Neurosci. 2015 Aug 10;18(9):1334–1341. doi: 10.1038/nn.4081

Figure 1. An optimized Cre recombinase dependent on GFP (CRE-DOGOPT).

Figure 1

(a) Schematic of CRE-DOGOPT action. (b) Schematic of chimeric constructs comprising CRE-DOGOPT. (c–e) Luciferase reporter assays in transfected 293T cells assessing CRE-DOGOPT specificity for all components of the system (c), specificity for different fluorescent proteins (d) and dependency on GFP level (e). n=18 per condition for (c and d), n=6 or 9 for (e). (see Online Methods for n definition). Results are representative of at least three independent transfections. Boxplots indicate minimum to maximum range.