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. Author manuscript; available in PMC: 2016 Apr 21.
Published in final edited form as: Nat Neurosci. 2015 Aug 10;18(9):1334–1341. doi: 10.1038/nn.4081

Figure 2. CRE-DOGOPT can be delivered to the mouse retina for retrofitting transgenic GFP lines.

Figure 2

(a–c) Electroporation of Tg(CRX-GFP) (a) and Tg(PROX1-GFP) (b) with plasmids encoding CRE-DOGOPT and the floxed reporter CALNL-DsRed (b) or ChAG-LtBFPL-mCherry (c). CAG-nlacZ, which expresses n-βgal, was added as an electroporation marker in (b). (a) Schematic of electroporation experiment. (b–c) CRE-DOGOPT induced floxed reporter expression in Tg(CRX-GFP) (b) and Tg(PROX1-GFP) (c) retinas, but not in GFP-negative retinas (b–c). Scale bar, 20 μm. Images representative of 3 or 4 retinas per condition. ONL, outer nuclear layer. INL, inner nuclear layer.