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. Author manuscript; available in PMC: 2016 Apr 21.
Published in final edited form as: J Cell Physiol. 2015 May;230(5):1075–1085. doi: 10.1002/jcp.24839

FIGURE 2. Phosphorylation of MATα2 and MATβ proteins in human HSCs.

FIGURE 2

A. Total cellular protein from day 0 and day 7 HSCs was immunoprecipitated with Pan-phospho antibody (IP: Phos) and immunoblotted for MATα2 or MATβ. α-tubulin from total protein was used for normalization. Results are densitometric analysis (mean ± S.E.) from four HSC preparations. *p<0.05 vs. day 0. B. Total cellular protein from day 0 and day 7 cells was subjected to SDS-PAGE in the absence or presence of phos-tag™ as described under Materials and Methods and immunoblotted for endogenous MATα2 (left panel) or endogenous MATβ (right panel). A representative image from three HSC preparations is shown. C. Human HSCs were transfected with MAT2A-DDK (left panel) or MAT2B-HA (right panel) constructs as described under Materials and Methods. Extracts from transfected cells at day 1 or day 7 were subjected to SDS-PAGE in the absence or presence of phos-Tag™. Immunoblotting for exogenous MATα2-DDK (left panel) or MATβ-HA (right panel) is shown. Data is representative of four independent experiments. Two representative experiments are shown.