Skip to main content
. Author manuscript; available in PMC: 2016 Apr 21.
Published in final edited form as: J Cell Physiol. 2015 May;230(5):1075–1085. doi: 10.1002/jcp.24839

FIGURE 3. Role of MAPK family in affecting MATα2 and MATβ phosphorylation.

FIGURE 3

A Recombinant MATα2 and MATβ proteins were subjected to in vitro kinase assay using active ERK1 and ERK2 enzymes as described under Material and Methods. Phosphorylated proteins were identified by immunoprecipitation-immunoblot using Pan-phospho antibody (Phos). Results are representative of three kinase assays. B. Cells treated with PD98059 were subjected to immunoprecipitation-immunoblotting or regular Western blotting to examine MATα2 or MATβ expression. Results are mean ± S.E. from three independent experiments. *p<0.05, ** p<0.005, †p<0.001, ¥p<0.01 vs. control. C. Cells were treated with PD98059 and chased for different times after cycloheximide treatment as described under Materials and Methods. Results represent the expression of MATα2 from three independent experiments expressed as percent of 0 hour control. Densitometric ratios were normalized to α-tubulin control at each time point and semi-logarithmic graphs were plotted to determine the half life of MATα2. *p<0.05, ¥ p<0.01 vs. control. D. Cells were treated as in ‘C’ and MATβ expression from three independent experiments is expressed as percent of 0 hour control. *p<0.05, ¥ p<0.01, †p<0.001 vs. control.