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. Author manuscript; available in PMC: 2017 Apr 1.

Published in final edited form as: Behav Brain Res. 2016 Jan 8;302:200–212. doi: 10.1016/j.bbr.2016.01.009

Fig. 4 — Western blot analysis of proteins extracted from olfactory bulb (OB), amygdala (A), periaqueductal grey (PAG) and hippocampus (HC) in male rats. Lanes 1–4 shows the mGluR1 immunoreactivity (monomer at 140 kDa and apparently the dimmer over 280 kDa) obtained using the anti-mGluR1 antibody (N-16; Santa Cruz Bio). Lanes 5–8 shows the mGluR1 anti-body immunoreactivity attenuated after incubation of N-16 pre-absorbed with the control peptide (sc-47131; Santa Cruz Bio.). Lower panel shows immunoreactivity of actin (lanes 1–8) obtained used as loading control. Represents the molecular weight size standard in kilo Dalton (KDa).

Inline graphic — Western blot analysis of proteins extracted from olfactory bulb (OB), amygdala (A), periaqueductal grey (PAG) and hippocampus (HC) in male rats. Lanes 1–4 shows the mGluR1 immunoreactivity (monomer at 140 kDa and apparently the dimmer over 280 kDa) obtained using the anti-mGluR1 antibody (N-16; Santa Cruz Bio). Lanes 5–8 shows the mGluR1 anti-body immunoreactivity attenuated after incubation of N-16 pre-absorbed with the control peptide (sc-47131; Santa Cruz Bio.). Lower panel shows immunoreactivity of actin (lanes 1–8) obtained used as loading control. Represents the molecular weight size standard in kilo Dalton (KDa).