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. Author manuscript; available in PMC: 2017 Apr 1.

Published in final edited form as: Behav Brain Res. 2016 Jan 8;302:200–212. doi: 10.1016/j.bbr.2016.01.009

Fig. 5 — Western blot analysis of proteins extracted from olfactory bulb (OB), amygdala (A), periaqueductal grey (PAG) and hippocampus (HC) in male rats. Lanes 1–4 shows the mGluR5 immunoreactivity (monomer at 150 KDa and apparently the dimmer over 250 KDa) obtained using the anti-mGluR5 antibody (AB5675; Millipore). Lanes 5–8 shows the mGluR5 anti-body immunoreactivity attenuated after incubation of AB5675 pre-absorbed with the control peptide (AG374; Millipore). Lower panel shows immunoreactivity of actin (lanes 1–8) obtained used as loading control. Represents the molecular weight size standard in kilo Dalton (kDa).

Inline graphic — Western blot analysis of proteins extracted from olfactory bulb (OB), amygdala (A), periaqueductal grey (PAG) and hippocampus (HC) in male rats. Lanes 1–4 shows the mGluR5 immunoreactivity (monomer at 150 KDa and apparently the dimmer over 250 KDa) obtained using the anti-mGluR5 antibody (AB5675; Millipore). Lanes 5–8 shows the mGluR5 anti-body immunoreactivity attenuated after incubation of AB5675 pre-absorbed with the control peptide (AG374; Millipore). Lower panel shows immunoreactivity of actin (lanes 1–8) obtained used as loading control. Represents the molecular weight size standard in kilo Dalton (kDa).