Expression of group I mGluR subtypes in the amygdala in ovariectomized female with high (OVX + EB), low (OVX) estradiol levels, and male rats. Representative immunoblots of mGluR1 (left panel) and mGluR5 (right panel) with its corresponding loading controls are shown in the upper panel. Histogram graphs of mGluR1 and mGluR5 protein levels normalized to actin are shown in the lower panel. OVX + EB rats showed higher mGluR1/actin ratio when compared to OVX and male rats. OVX showed higher mGluR1/actin ratio than male rats (OVX + EB, n = 6; OVX, n = 6; Male, n = 6). No differences were detected for mGluR5/actin ratio between the experimental groups (OVX + EB, n = 5; OVX, n = 6; male, n = 6). Values represent mean ± SEM. * = p ≤ 0.05.