. 2015 Sep 16;5(3):e1066062. doi: 10.1080/2162402X.2015.1066062

Table 1.

Results of flow cytometry analysis.

	Pre-Dex			Post-Dex
Parameter measured	Mean	StDev	n =	Mean	StDev	n =	% change	p value
CD3 T cells per μL	848	401	30	370	207	32	−56.3	<0.001
CD4 T cells per μL	551	199	31	236	149	33	−57.2	<0.001
CD4 % of CD3 T cells	65.4	10.8	32	57.6	12.8	35	−11.9	<0.001
CD8 T cells per μL	246	212	30	124	103	31	−49.8	<0.001
CD8 % of CD3 T cells	27.1	10.3	32	33.7	13.4	34	24.4	<0.001
CD4 to CD8 T cell ratio	3.0	1.9	32	2.3	1.7	34	−25.3	<0.001
Tregs per μL	24.0	11.9	31	9.7	6.5	33	−59.3	<0.001
Treg % of CD4⁺ T cells	4.2	1.3	35	4.2	1.4	35	−1.2	0.755
Ki67⁺ % of Tregs	21.3	6.9	35	38.3	13.0	35	79.5	<0.001
Ki67⁺ % of non-Treg CD4 T cells	3.1	1.4	35	4.8	2.4	35	53.1	<0.001
ICOS⁺ % of Tregs	35.2	15.3	35	46.7	15.7	35	32.4	<0.001
ICOS⁺ % of non-Treg CD4 T cells	6.5	5.0	35	7.0	5.2	35	8.4	0.121
effector (CD38^hiHLA-DR^hi) CD8 T cells per μL	4.4	3.9	30	2.3	1.3	31	−46.5	0.003
effector % of CD8 T cells	1.9	1.5	35	2.5	1.9	35	33.4	<0.001
Bcl2^lo % of effector CD8 T cells	66.5	17.0	35	64.1	18.1	35	−3.6	0.293
Ki67⁺ % of effector CD8 T cells	63.3	17.2	35	69.7	17.1	35	10.0	0.009
ICOS⁺ % of effector CD8 T cells	27.9	14.2	35	28.8	13.7	35	3.3	0.608
Bcl2^lo % of CD8 T cells	3.8	2.2	35	3.6	2.6	35	−5.9	0.527
Ki67⁺ % of CD8 T cells	2.0	1.5	35	2.9	2.0	35	45.7	0.001
ICOS⁺ % of CD8 T cells	2.1	3.8	35	1.7	1.9	35	−20.7	0.353
CD8 T cells to Treg ratio	10.7	6.3	30	17.0	13.5	31	59.2	<0.001
DC (HLA-DR⁺ Lin^-) % of PBMC	0.4	0.2	15	0.3	0.2	15	−31.4	0.035
HLA-DR⁺ lin^- BDCA1⁺ % of total PBMC	0.159	0.120	15	0.085	0.102	15	−46.3	0.024
HLA-DR⁺ lin^- BDCA2⁺ % of total PBMC	0.147	0.095	15	0.048	0.053	15	−67.2	0.001
HLA-DR⁺ lin^- BDCA3⁺ % of total PBMC	0.028	0.019	15	0.022	0.015	15	−21.9	0.279
BDCA1⁺ % of DC	35.6	19.8	15	21.8	18.8	15	−38.8	0.023
BDCA2⁺ % of DC	32.6	13.7	15	17.2	14.4	15	−47.1	0.008
BDCA3⁺ % of DC	5.6	2.4	15	12.0	8.4	15	116.2	0.003
BDCA1⁺ Dc to BDCA²⁺ DC ratio	2.1	2.6	15	2.2	2.3	15	5.3	0.884
BDCA1⁺ DC CD40 MFI	43.0	32.3	15	75.6	58.3	15	75.7	0.005
BDCA1⁺ DC CD83 MFI	78.7	34.1	15	64.8	27.8	15	−17.7	0.188
BDCA1⁺ DC CD80 MFI	110	42	15	135	69	15	22.7	0.036
BDCA2⁺ DC CD40 MFI	27.0	24.1	15	17.0	19.3	15	−37.0	0.138
BDCA2⁺ DC CD83 MFI	34.7	13.8	15	35.8	12.7	15	2.9	0.831
BDCA2⁺ DC CD80 MFI	129	25	15	241	440	15	86.4	0.344
BDCA3⁺ DC CD40 MFI	29.5	70.9	15	25.2	84.3	15	−14.5	0.744
BDCA3⁺ DC CD83 MFI	46.6	58.2	15	37.5	30.2	15	−19.5	0.408
BDCA3⁺ DC CD80 MFI	198	45	15	229	96	15	15.8	0.118

List of leukocyte parameters retrospectively examined for changes in response to Dex. For timing of blood sample withdrawal and Dex administration, see Figure 1A. “% change” refers to difference in mean values between “pre-Dex” and “post-Dex” timepoints. P values were calculated using the paired t-test.