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. 2015 Oct 29;5(4):e1100790. doi: 10.1080/2162402X.2015.1100790

Figure 2.

Figure 2.

Effect of HER2 overexpression and knockdown on the level of HLA-ABC expression in breast cancer cells. (A). MCF7 and MCF7-HER18 cells were double-stained with APC-conjugated anti-HLA-ABC antibody and trastuzumab plus FITC-conjugated anti-human IgG antibody and subjected to flow cytometry analysis. Upper panel, flow cytometric data on HLA-ABC expression vs. HER2 expression in MCF7 and MCF7-HER18 cells in contour plots. Lower panel, MFI values of HLA-ABC and HER2 expression (left) and the percentages of cells expressing HLA-ABC and HER2 (right) in MCF7 and MCF7-HER18 cells. (B). The indicated HER2-overexpressing human breast cancer cells were transfected with one of two different HER2-specific siRNAs or control siRNA using Lipofectamine for 72 h in culture. The cells were double-stained for flow cytometry analysis of HLA-ABC and HER2 and the data were plotted as described in (A). * p < 0.05 compared with corresponding control.