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. 2015 Oct 29;5(4):e1105429. doi: 10.1080/2162402X.2015.1105429

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© 2016 The Author(s). Published with license by Taylor & Francis Group, LLC

This is an Open Access article distributed under the terms of the Creative Commons Attribution-Non-Commercial License http://creativecommons.org/licenses/by-nc/3.0/, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted.

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Figure 3. — Cross-presentation of modified SIINFEKL analogs on MHC-I adsorbed or not adsorbed onto the viral capsid. (A) C57BL/6 fresh spleenocytes were incubated with SIINFEKL, the amino caproic acid-containing SIINFEKL-AHX-polyK, the C-terminus-extended SIINFEKL-polyK or the N-terminus-extended polyK-SIINFEKL. Cross presentation was determined with APC anti-H-2K^b bound to SIINFEKL or isotype control antibodies. (B) Similar to (A), spleenocytes were infected with of OVA-PeptiCRAd, or incubated with peptides SIINFEKL or polyK-SIINFEKL. The data are shown as the mean ± SD (n=2). Significance was assessed using the unpaired student´s t-test; ** p < 0.01, *** p < 0.001.