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. 2015 Nov 10;5(3):e1100791. doi: 10.1080/2162402X.2015.1100791

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© 2016 The Author(s). Published with license by Taylor & Francis Group, LLC

This is an Open Access article distributed under the terms of the Creative Commons Attribution-Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted.

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Figure 2. — Evaluation of the functional capacity of innate immune cells upon short-term stimulation by multi-parametric flow cytometry. After 5 h of culture in presence of medium or activators, brefeldin A being added after 1 h, we assessed the functional capacity of different innate immune cells characterized as described in Fig. 1B. Dot plots present cytokine intracytoplasmic staining to evaluate production of IFNγ/TNFα by NK cells or IFNα/TNFα by pDC as well as IL-12p40/TNFα by mDC (BDCA-3⁺, BDCA-1⁺) or monocyte (CD14⁺CD16^+/−, CD14^lowCD16⁺) subsets. (A) The efficiency of different activators (TLR7/8 ligand (R848), Type-I IFN (IFNα−2b), TLR3 ligand (poly(I:C)), TLR9 ligand (CpG-B)) was compared to medium condition in whole blood assay. (B) Comparison of results obtained after 5 h of stimulation with R848 (10µg/mL) on whole blood or PBMC from the same donor.