Table 3.
Marker | Fluorochrome | Clone | Manufacturer |
---|---|---|---|
A : Panel used for T cell functionality assay | |||
Violet laser | |||
IFNγ | Brilliant violet 421™ | 4S.B3 | Biolegend |
CD14 | BD Horizon™ V500 | M5E2 | BD Biosciences |
CD15 | BD Horizon™ V500 | HI98 | BD Biosciences |
CD19 | BD Horizon™ V500 | HIB19 | BD Biosciences |
CD8+ | Brilliant violet 570™ | RPA-T8 | Biolegend |
Blue laser | |||
TCRγ9 | FITC | B3 | BD Biosciences |
IL-17A | PerCP-Cy5.5 | N49-653 | BD Biosciences |
Yellow laser | |||
CD4 | PE | SK3 | BD Biosciences |
CD45RA | ECD | 2H4LDH11LDB9 | Beckman Coulter |
IL-2 | PE-Cy7 | MQ1-17H12 | BD Biosciences |
Red laser | |||
IL-21 | Alexa 647 | 3A3-N2.1 | BD Biosciences |
TNFα | Alexa 700 | MAb11 | BD Biosciences |
CD3 |
APC-H7 |
SK7 |
BD Biosciences |
B: Panel used for “Innate Immunity” assay | |||
Violet laser | |||
IFNγ | Brilliant violet 421™ | 4S.B3 | Biolegend |
CD3 | BD Horizon™ V500 | UCHT1 | BD Biosciences |
CD14 | BD Horizon™ V500 | M5E2 | BD Biosciences |
CD15 | BD Horizon™ V500 | HI98 | BD Biosciences |
CD19 | BD Horizon™ V500 | HIB19 | BD Biosciences |
CD56 | Brilliant violet 570™ | HCD56 | Biolegend |
Blue laser | |||
IFNα | FITC | LT27:295 | Miltenyi Biotec |
CD11c | PerCP-Cy5.5 | Bu15 | Biolegend |
Yellow laser | |||
IL12p40/70 | PE | C11.5 | BD Biosciences |
CD16 | ECD | 3G8 | Beckman Coulter |
CD1c | PE-Cy7 | L161 | Biolegend |
Red laser | |||
BDCA-2 | APC | AC144 | Miltenyi Biotec |
BDCA-3 | AD5-14H12 | Miltenyi Biotec | |
TNFα | Alexa 700 | MAb11 | BD Biosciences |
HLA-DR | APC-H7 | G46-6 | BD Biosciences |