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. 2015 Nov 10;5(3):e1100791. doi: 10.1080/2162402X.2015.1100791

Table 3.

Staining panels used for flow cytometry analyses.

Marker Fluorochrome Clone Manufacturer
A : Panel used for T cell functionality assay
  Violet laser    
IFNγ Brilliant violet 421™ 4S.B3 Biolegend
CD14 BD Horizon™ V500 M5E2 BD Biosciences
CD15 BD Horizon™ V500 HI98 BD Biosciences
CD19 BD Horizon™ V500 HIB19 BD Biosciences
CD8+ Brilliant violet 570™ RPA-T8 Biolegend
  Blue laser    
TCRγ9 FITC B3 BD Biosciences
IL-17A PerCP-Cy5.5 N49-653 BD Biosciences
  Yellow laser    
CD4 PE SK3 BD Biosciences
CD45RA ECD 2H4LDH11LDB9 Beckman Coulter
IL-2 PE-Cy7 MQ1-17H12 BD Biosciences
  Red laser    
IL-21 Alexa 647 3A3-N2.1 BD Biosciences
TNFα Alexa 700 MAb11 BD Biosciences
CD3
APC-H7
SK7
BD Biosciences
B: Panel used for “Innate Immunity” assay
  Violet laser    
IFNγ Brilliant violet 421™ 4S.B3 Biolegend
CD3 BD Horizon™ V500 UCHT1 BD Biosciences
CD14 BD Horizon™ V500 M5E2 BD Biosciences
CD15 BD Horizon™ V500 HI98 BD Biosciences
CD19 BD Horizon™ V500 HIB19 BD Biosciences
CD56 Brilliant violet 570™ HCD56 Biolegend
  Blue laser    
IFNα FITC LT27:295 Miltenyi Biotec
CD11c PerCP-Cy5.5 Bu15 Biolegend
  Yellow laser    
IL12p40/70 PE C11.5 BD Biosciences
CD16 ECD 3G8 Beckman Coulter
CD1c PE-Cy7 L161 Biolegend
  Red laser    
BDCA-2 APC AC144 Miltenyi Biotec
BDCA-3   AD5-14H12 Miltenyi Biotec
TNFα Alexa 700 MAb11 BD Biosciences
HLA-DR APC-H7 G46-6 BD Biosciences