Fig. S2.

SiMPull reveals a lack of heteromerization between TREK1 and TASK1 or TASK3, but clear heteromerization between TASK1 and TASK3. (A) TIRF images of immobilized single molecules of HA-TREK1–mediated pull-down of GFP-TREK1 (Left), GFP-TASK1 (Middle), or GFP-TASK3 (Right). (B) Summary of HA-TREK1 pull-down showing much more efficient pull-down of TREK1 compared with TASK1 or TASK3. Dilution factors describe how much lysate was diluted before application onto a coverslip. (C) Representative images showing that HA-TASK3 is able to pull down GFP-TASK1 or GFP-TASK3 with comparable efficiency. (D) Summary of HA-TASK1 pull-down of GFP-TASK1 and GFP-TASK3 compared with controls with GFP-TASK1 or HA-TASK1 alone.