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. 2016 Mar 28;113(15):4218–4223. doi: 10.1073/pnas.1513093113

Fig. 2.

Fig. 2.

GLK1/2 control stomatal opening. (A) Stomatal aperture of epidermal fragments from the plants under dark conditions, in which plants were incubated 1 d before measurements (dark), and light conditions (light). (B) Stomatal aperture of epidermal fragments incubated with 0, 1, and 20 µM ABA under white light for 2.5 h. The average of three independent experiments is shown. Approximately 150 stomata in total (n = 3 independent experiments, 50 stomata per experiment) were analyzed in each line. Error bars represent SD. (C) Thermal images of WT (Upper) and GLK1sx (Lower) plants grown on MS medium, showing the higher temperature of GLK1sx plants. (D) Water loss in WT and GLK1sx plants for the evaluation of transpiration rate. The average of three biological replicates (five to seven plants per replicate) is shown. Error bars represent SD.