Table S3.
Primer name | Target gene name | Sequences (5′– 3′) |
For semi-qRT-PCR | ||
AT2G20570-RT-s | GLK1 | CCTCTTCAGCTTCTTCCAAGAAC |
AT2G20570-RT-as | GLK1 | GCGGTGCTCTAAATCTCGTAG |
AT5G44190-RT-s | GLK2 | ACAAAACGGTGCGTCGAG |
AT5G44190-RT-as | GLK2 | ATGCCATACCATATCCCTGTG |
PP2AA3-F | PP2Aa3 | GCCATTGTAGAACTTGCTGAAGACAGG |
PP2AA3-R | PP2Aa3 | TCCACCAAGCATGGCCGTATCATG |
For RT-PCR | ||
5G46240Rf1 | KAT1 | GACGCTGAGTATTTCCCACCAA |
5G46240Rr1 | KAT1 | GAAGTCCACTGCTCCTGACA |
4G18290fRT2 | KAT2 | GAAGCTCCTACTGACCTCTACAT |
4G18290rRT2 | KAT2 | TCTCTCCAAATGCATCGCCAACAA |
2G26650Rf1 | AKT1 | TGCGCTCCATACCGCTGTAT |
2G26650Rr1 | AKT1 | ATCCGCACCTTGCTCCAGAA |
1G65480Rf1 | FT | CTGCTACAACTGGAACAACCTTTG |
1G65480Rr1 | FT | TTTGCCTGCCAAGCTGTCGAA |
4G14480fRT | BLUS1 | TTTTCTGATTACGAGATCAATACGA |
4G14480rRT | BLUS1 | GCTTTTGAGAACTTCTTGTTACCC |
2G18960Rf1 | AHA1 | GCTTGGGCCAGCTTGTTTGA |
2G18960Rr1 | AHA1 | TTTGGCTGCAGACCGTGCAA |
4G30190Rf1 | AHA2 | GCAAGCTAAGAGAAGAGCTGAGA |
4G30190Rr1 | AHA2 | ACAGTGTAGTGACTGGGAGTTTCA |
AT2G24520qRTf | AHA5 | GGCTGTTGCAAGACAGGAA |
AT2G24520qRTr | AHA5 | CGGAGGATCAAAAAGAGGTAAA |
1G13320Rf2 | PP2Aa3 | GACCAAGTGAACCAGGTTATTGG |
1G13320Rr2 | PP2Aa3 | TACTCTCCAGTGCCTGTCTTCA |
For plasmid construction | ||
AT2G20570N | GLK1 | gATGTTAGCTCTGTCTCCGGCGACAAGAGA |
AT2G20570.1SC | GLK1 | TCAGGCACAAGACGCGGTCGGAGGAACCTC |
AT5G44190N | GLK2 | gATGTTAACTGTTTCTCCGGCTCCAGTACT |
AT5G44190.1SC | GLK2 | TCAAGGAAGAGGAGGAACATTAGAAACTCC |
At1g22690pF1725 | GC1 | GGGGACAACTTTGTATAGAAAAGTTGCTACAAGAAGAGTAAAGATTCAGTAAC |
At1g22690pR | GC1 | GGGGACTGCTTTTTTGTACAAACTTGGATTTCTTGAGTAGTGATTTTGAAGTAG |
AT5G46240pF2700 | KAT1 | GGGGACAACTTTGTATAGAAAAGTTGCCAGAAAAGAAAAAAAATTGTAGTGGA |
AT5G46240pR | KAT1 | GGGGACTGCTTTTTTGTACAAACTTGGCTTTTTTTCTTTTTGGTTGAGAATTTG |
qRT-PCR, quantitative RT-PCR.