Fig. 3.

Increased CDA expression reduces dC levels and increases [³H]CFA accumulation by CEM leukemia cells. (A) Western Blot for CDA expression in CEM cells engineered to express EYFP or CDA. (B) LC-MS/MS-MRM measurements of dC levels in culture supernatants from these cells incubated for the indicated times with 5 µM [U-¹³C₉,¹⁵N₃]dC. n = 3. (C) [³H]CFA (9.25 kBq) uptake (over 1 h) in the isogenic panel described in A, after 6 h of incubation in medium supplemented with dC (5 µM) ± DI-82 (1 µM). N.S., nonsignificant; ***P < 0.001. Results are representative of three independent experiments (n = 3 for each group).