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. 2015 Mar 11;40(8):1910–1918. doi: 10.1038/npp.2015.40

Figure 5.

Figure 5

ICV injection of C8X can increase motivation to obtain nicotine in nicotine-seeking behavior, and repeated ICV injections of C8X does not alter peripheral nicotine metabolism. (a) Mean active lever presses (±SEM) for the first four daily extinction sessions (C8X: 40±6, 28±4, 21±2, and 23±3; ACSF: 39±4, 26±4, 26±3, and 25±2) were not different between C8X (n=24) and ACSF (n=21) groups (Supplementary Figure). Animals then underwent multiple extinction sessions the same day until they reached extinction criteria. Data shown is the proportion of C8X and ACSF rats that met extinction criteria over these extinction sessions where C8X-treated animals needed more sessions to meet criteria. During nicotine reinstatement testing, mean active lever presses between C8X and ACSF were not different after (b) IV (C8X: n=17, ACSF: n=18) or (c) SC (C8X: n=24, ACSF: n=21) nicotine priming injections at all doses tested. Mean active lever presses between C8X and ACSF after 60 μg/kg/IV approached significance (p=0.06). *p<0.05, saline (0 μg/kg nicotine) vs 60 and 150 μg/kg/SC for both C8X and ACSF. There was no difference in plasma (d) nicotine and (e) cotinine between ACSF and C8X treatments after four nicotine infusions were given in the operant SA chamber (7.5 μg/kg/infusion) at the end of NSA in a subset of rats (n=8 for both C8X and ACSF). Data shown as mean±SEM.