Figure 5.

Inherent impulsive action predicts synaptic protein expression in mPFC. (a) Following 1-CSRT task training and phenotypic identification (cohort 5), the mPFC was collected for biochemical analysis. Immunoblot for the 5-HT_2AR and cadherin loading control was performed using crude synaptosomal protein from the mPFC (inset, representative blot). Densitometric quantitation revealed elevated 5-HT_2AR protein expression in HI (n=7, black bar) relative to LI rats (n=7, white bar; p<0.05). (b) Immunoprecipitation (IP) of mPFC crude synaptosomes with α-PSD95 antibody followed by immunoblots (IB) for 5-HT_2AR and PSD95 (right, representative blots) revealed an increased 5-HT_2AR/PSD95 association in HI (n=4, black bar) relative to LI rats (n=3, white bar; p<0.05). (c) There was a correlation between premature responses in the 1-CSRT task and densitometric quantitation of 5-HT_2AR/PSD95 association (r=0.822; p<0.05). Arbitrary units (AU) of normalized densitometry are presented (see Materials and Methods).