Figure 2.

Increased firing of Glp-1r^−/− septal neurons. (a) The time to fire an AP is delayed in Glp-1r^−/− (total of 23 cells from 10 animals) compared with Glp-1r^+/+ (total of 21 cells from 11 animals) neurons (P=0.02). (b) The number of APs at twice threshold was elevated in Glp-1r^−/− compared with Glp-1r^+/+ controls (P=0.04). (c) Representative responses of Glp-1r^+/+ (left) and Glp-1r^−/− (right) neuron, respectively, to 600 ms depolarizing current steps at 100 and 150 pA. (d) Responses (number of APs evoked by 600 ms stimulus) of Glp-1r^+/+ and Glp-1r^−/− cells across a range of step current injections from 10 to 150 pA revealed an increased activity of Glp-1r^−/− neurons (two-way repeated measures ANOVA × genotype effect: F(1, 43)=6.48, P=0.01). *P<0.05, **P<0.01.