Table 1. Primer sequences used for qPCR experiments on N. tabacum.
Gene (N. tabacum) | qPCR primer sequence (5’-3’) /degenerate primer sequence (5’3’) where required |
---|---|
CER3 FW | GCGCAATGGCTCTCCCAAGCT/AAYGARGCNYTNAAYGGNGGN |
CER3 RV | GGCGGAACGCTCTTTGTCGACA/NGCNCCNACYTCRTGRTG |
CER5 FW | GGCTCGAGGAGCTTGGTGGA |
CER5 RV | TCCGGTGAGGAGTGAAACCGCA |
CER6 FW | CTCCGGTTACCTGCCGAGTCCC |
CER6 RV | GCAGGAGGCAAGCACGTTTCTTCTC |
EF1α FW | TCTCCAGGAGGCACTCCCTGG |
EF1α RV | TGATGATGACCTGGGAGGTGA |
PDF2 FW | AGGCTTGCAGCCGGTGAATGG |
PDF2 RV | TTGTCGCAGCCGACCTTCGC |