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. 2016 Apr 21;11(4):e0152836. doi: 10.1371/journal.pone.0152836

Table 1. Primer sequences used for qPCR experiments on N. tabacum.

Gene (N. tabacum) qPCR primer sequence (5’-3’) /degenerate primer sequence (5’3’) where required
CER3 FW GCGCAATGGCTCTCCCAAGCT/AAYGARGCNYTNAAYGGNGGN
CER3 RV GGCGGAACGCTCTTTGTCGACA/NGCNCCNACYTCRTGRTG
CER5 FW GGCTCGAGGAGCTTGGTGGA
CER5 RV TCCGGTGAGGAGTGAAACCGCA
CER6 FW CTCCGGTTACCTGCCGAGTCCC
CER6 RV GCAGGAGGCAAGCACGTTTCTTCTC
EF1α FW TCTCCAGGAGGCACTCCCTGG
EF1α RV TGATGATGACCTGGGAGGTGA
PDF2 FW AGGCTTGCAGCCGGTGAATGG
PDF2 RV TTGTCGCAGCCGACCTTCGC