Table 1. Key flux ratios compared with previous studies using ¹³C metabolic flux analysis.

Flux ratioa	Correlation with increased ethanol yields		Corresponding genes	Correlation with gene expression ratios
	omFBA	Previous studies [32, 33, 42]		omFBA	Previous studies[31]
PGI/G6PDH2	Positive	Positive	YBR196C/YNL241C	No correlationb	No correlation
FBA/TKT1	Positive	Positive	YKL060C/YPR074C	No correlation	No correlation
ENO/PPCK	Negative	Negative	YGR254W/YKR097W	No correlation	No correlation
PYK/PYRDC	Negative	Negative	YAL038W/YLR044C	No correlation	No correlation

PGI, glucose-6-phosphate isomerase; G6PDH2, glucose 6-phosphate dehydrogenase; FBA, fructose-bisphosphate aldolase; TKT1, transketolase; ENO, enolase; PPCK, phosphoenolpyruvate carboxykinase; PYK, pyruvate kinase; PYRDC, pyruvate decarboxylase.

^aAll the abbreviations were from the iND750 model.

^bNo correlation was defined as the cases in which the correlation coefficient (absolute value) was smaller than 0.2.