Table 1. Concentration and purity of total RNA extracted from milk fat, whey and somatic cells.
Cow number | Concentration (ng/ml) | OD260/280 | OD260/230 | RNA integrity number | |
---|---|---|---|---|---|
Milk fat | 12 | 242.3 | 2.1 | 2.1 | 2.2 |
16 | 173.5 | 2.1 | 2.1 | 2.5 | |
710 | 125 | 2.2 | 2.3 | NA | |
7134 | 152.8 | 2.1 | 2.1 | 3.2 | |
Milk whey | 12 | 147.5 | 2 | 1.6 | 2.7 |
16 | 73.1 | 2 | 0.7 | 2.6 | |
710 | 49.9 | 1.9 | 1.1 | NA | |
7134 | 74.8 | 1.9 | 1.3 | 2.7 | |
Milk cell | 12 | 145.2 | 2.1 | 1.8 | 7.3 |
16 | 136 | 2.2 | 1.9 | 8.3 | |
710 | 264.2 | 2.1 | 1.9 | 8 | |
7134 | 186.6 | 2.1 | 2 | 6.7 |
Note: NA = Not available (not determined by Bioanalyzer 2100).