Table 1. Germline transmission rates of chimeras made with CRISPR-modified PGCs.
| Cell line | Chimera | # progeny screened | % germline transmission |
|---|---|---|---|
| 1783–1 | 20649 | 29 | 0 |
| 1783–3 | 20622 | 305 | 8.6 |
| 1783–6 | 20626 | 19 | 0 |
| 20638 | 175 | 16 | |
| 20643 | 35 | 0 | |
| 1783–9 | 20455 | 37 | 0 |
| 20457 | 25 | 0 | |
| 20492 | 27 | 0 | |
| 20499 | 40 | 5 | |
| 20642 | 149 | 15 | |
| 1783–10 | 20619 | 130 | 96 |
| 20623 | 34 | 12 | |
| 20646 | 154 | 36 |
Chimeras (bird IDs are listed) were germline tested by screening progeny for EGFP expression. % germline transmission was calculated as %EGFP+ progeny x 2, since EGFP was heterozygous in the PGCs injected.