Fig 5.
P2 increases IL-12 secretion by LPS stimulated PBMCs: (A) 3×105 human PBMCs were either left overnight unstimulated (UN) or stimulated with LPS (4×10−3 μM), LPS+0.3 μM of anti-IL10 (LPS+aIL10), LPS and P1 at 9.0 μM, LPS and P2 at 8.2 μM, respectively. Supernatants were measured for the presence of IL-12p40 by ELISA. (B) 1×105 of human PBMCs were either unstimulated or stimulated with LPS or LPS with P3, P4 and different concentration of P2 overnight, IL-12 p40 from supernatants were measured by ELISA. (C) 1×106 PBMCs were stimulated in the presence of 100 ng/mL of LPS, 5 μg/mL of P2 or P3 for 72 hours. Cells were stimulated with stimulation cocktail (ebioscience) in the final 6 hours and stained with anti-CD3, anti-CD8 and intracellularly stained with IFNγ. FACS were performed and CD3+CD8+IFNγ+ were analyzed with Flowjo.