Table 2. The time limits for conventional carbon dioxide-anesthesia for various aquatic species.
| Fish species | Individual number | CO2 level for anesthesia initiation* (%) | CO2 level for maintenance of anesthesia (%) | Lethal time(min.) |
|---|---|---|---|---|
| Bigfin reef squid | No.1 | 5.3 | 7.0 | 10 |
| No.2 | 4.2 | 7.0 | 15 | |
| White-lined rockcod | No.3 | 6.8 | 8.0 | 10 |
| No.4 | 5.8 | 8.0 | 20 | |
| Chicken grunt | No.5 | 4.0 | 5.0 | 10 |
| No.6 | 2.9 | 5.0 | 10 | |
| Japanese scad | No.7 | 4.3 | 7.0 | 10 |
| No.8 | 6.0 | 7.0 | 15 | |
| Red seabream | No.9 | 8.9 | 10.0 | 25 |
| No.10 | 7.3 | 10.0 | 15 |
* Carbon dioxide concentration judged by macroscopic observation when it was in the anesthetic condition that the condition of the fish was equivalent to for the second phase from the first phase of the depth of anesthesia in human general anesthesia.