Table 1. Oligonucleotides inserted into the BbsI site of pSpCas9(BB)-2A-GFP.

sgRNA sequences are indicated in bold and preceded by a G nucleotide to improve transcription [29].

sgRNA	Orientation	Sequence
S1	fwd	CACCGAGATGAGCTCAGACCCTGGG
S1	rev	AAACCCCAGGGTCTGAGCTCATCTC
S2	fwd	CACCGAATCTCTAAGTAGGGCTCAA
S2	rev	AAACTTGAGCCCTACTTAGAGATTC
S3	fwd	CACCGTAATTGCATGGAGTGTCGTG
S3	rev	AAACCACGACACTCCATGCAATTAC
S4	fwd	CACCGGCCTCTATCTTGCGTAGTCC
S4	rev	AAACGGACTACGCAAGATAGAGGCC