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. 2016 Apr 21;11(4):e0153926. doi: 10.1371/journal.pone.0153926

Fig 5. Mechanical strain induces IL-8 expression.

Fig 5

Fibroblasts from non-asthmatic (filled circles) or asthmatic (open circles) donors were cultured in the absence or presence of cyclical mechanical strain for 48 and 96h. A) IL-8 mRNA expression at 48h was assessed by RT-qPCR with the data normalized to GAPDH and displayed relative to the median of the unstrained non-asthmatic group using the ΔΔCT method. B) Release of IL-8 protein into the cell culture supernatants was assessed after 96 h by ELISA and normalised for cell number. The data were analysed using Wilcoxon’s signed rank test. Results shown represent data for fibroblasts from non-asthmatic (n = 6) or asthmatic (n = 11) donors for both the RT-qPCR and ELISA.