Fig 5. Conformational characteristics of EK21 peptide fragment derived from N-terminus of the F1 loop.

(panel A) Far UV CD spectra of EK21 peptide in free buffer solution (solid line) and in solutions containing either 50 mM DPC (solid line with black squares) or 50 mM SDS (solid line with white squares). (penal B) Bar diagram showing secondary chemical shifts of αH resonances of residues of EK21 in solutions containing SDS micelles. (panel C) Selected section of ¹H-¹H two-dimensional NOESY spectrum of EK21 peptide obtained in aqueous solutions containing 200 mM perdeuterated SDS.