Fig 7. Subcellular localization analysis by transient expression of CmLOX10 and CmLOX13 fusion constructs in tobacco leaves.

Tobacco leaves were infiltrated with Agrobacterium tumefaciens carrying the LOX10-GFP (A) and LOX13-GFP constructs (B). Both green fluorescent signals and spontaneous red fluorescent signals of chloroplasts were detected using Zeiss LSM510 confocal microscope excited with a 488-nm Ar laser line. Merge is the computed overlay of the two fluorescence images. Reference bar is 20 μm.