Fig 2. Experimental scheme.
Ten lactating cows were immunized by the intramuscular route with ovalbumin (OVA) and hen egg lyzozyme (HEL) emulsified in the MontanideTM ISA 61 VG adjuvant (Seppic) on days 0 and 30. Blood samplings were carried out to monitor the cell-mediated immune response with a whole-blood antigen stimulation assay (WBA). The cows were then challenged by infusion of either OVA, E. coli LPS, or both agents into the MG through the teat canal on day 60 post-immunization. Milk samples were taken during the follow-up period to monitor the inflammatory response.