Fig 8. Dependences of the hydrodynamic radius (R_h) on the portion of the denatured protein (γ_den) for aggregation of BSA at (A) 60°C, (B) 65°C, (C) 70°C and (D) 80°C.

The values of γ_den were calculated from Eq 4. For each temperature parameters B, k_1,den and k_2,den indicated in corresponding panels of Fig 2 were used. The R_h vs γ_den plots at 65°C and 70°C (panels B and C) were used for determination of the hydrodynamic radius of the primary aggregates (R_h,1).